The Centre for Phenogenomics
The Centre for Phenogenomics

Model Production Service Fees

The following information is an estimate of costs associated with mouse model generation.  A detailed cost estimate can be developed based on your specific project.  All fees are approximate and may change based on procedural/experimental outcomes. Services can be provided à la carte or as a full service from design to quality control.

CRISPR/Cas9 Mouse Model Generation

Fees for microinjection do not include screening P founders, breeding P founders for germline transmission, screening N1 progeny, or animal export and shipping charges. Microinjection fees change depending on mouse costs, which are a function of both strain and source. We have successfully used C57BL/6NCrl, C57BL/6J, 129S6/SvEv, ICR, and NOD strain backgrounds. Please enquire for fees for development and validation of genotyping assays.

Swipe to view

gRNA synthesis & preparation Canadian academic International academic
Indel allele (1 gRNAs) $520.00 $870.00
Deletion allele (2 gRNAs) $605.00 $980.00
Point mutation (1 gRNA + oligo) $720.00 $1,130.00
Deletion allele (4 gRNAs) $775.00 $1,200.00

Swipe to view

Gene annotation & allele design Canadian academic International academic
Includes gRNA & oligo design $180.00* $288.00*

*free of charge if mouse is made at TCP

Swipe to view

Microinjection Canadian academic International academic
Microinjection of C57BL/6 zygotes/attempt $1,975 $3,150

Swipe to view

Screening P founders, up to 25 founders including assay design Canadian academic International academic
By end-point PCR1 $965.00 $1,570.00
By PCR-Sequencing2 $1,205.00 $1,970.00

1deletion alleles; 2indel & point mutant alleles

Swipe to view

Allele sequencing Canadian academic International academic
PCR and subcloning1 $2,410.00 $3,295.00
Direct PCR2 $1,170.00 $1,610.00

1indel alleles; 2deletion & point mutant alleles

Gene Targeting

Swipe to view

Service Canadian academic International academic
Vector design construction & preparation for electroporation contact us contact us
Electroporation & isolation of antibiotic-resistant ES cell clones/attempt $1,800.00 $2,880.00
Screening ES cell clones contact us contact us
Expansion of positive ES cells/ clone $80.00 $128.00
ES cell chromosome QC/clone $140.00 $224.00
ES cell chromosome QC, rush (up to 6 clones) $780.00 $1,248.00

Generation of Chimeras

Swipe to view

ES cell services Canadian academic International academic
Expansion of ES cells/clone (15% discount/additional clone from same source at same time) $300.00 $480.00
ES cell pathogen test (up to 6 clones) $250.00 $400.00
Preparation of ES cell clones for aggregation or microinjection/clone $120.00 $192.00
ES cell chromosome QC/clone $140.00 $224.00
ES cell chromosome QC, rush (up to 6 clones) $780.00 $1,248.00

Fees for Generation of Chimeras do not include export and shipping charges

Swipe to view

Generation of chimeras Canadian academic International academic
By outbred morula aggregation or microinjection/attempt (up to 2 clones) $2,700.00 $4,320.00
By C57BL/6 morula microinjection/attempt (up to 2 clones) $2,800.00 $4,480.00
By tetraploid complementation assay/attempt (up to 2 clones, for dissections at mid-gestation) $2,600.00 $4,160.00

Swipe to view

Additional services Canadian academic International academic
Germline transmission testing contact us contact us
Screening GLT offspring contact us contact us

Transgenic Mouse Production

Fees for pronuclear microinjection do not include preparation of the transgenic construct for microinjection, screening F0 founders, export fees, or shipping charges.

Swipe to view

Service Canadian academic International academic
Transgene vector design, construction & preparation for microinjection contact us contact us
Pronuclear microinjection into CD-1 zygotes from ~15-20 embryo donors and transfer to ~8 recipients /attempt $2,500.00 $4,000.00
Pronuclear microinjection into FVB/N, C57BL/6N or C57BL/6J zygotes from ~15-20 embryo donors and transfer to ~8 recipients /attempt $2,800.00 $4,480.00
Screening F0 founders contact us contact us
Germline transmission testing contact us contact us
Transgene copy number assessment contact us contact us

Derivation of Novel ES Cell Lines

Fees do not include purchase of wild type female embryo donors, animal housing during superovulation and mating, pathogen testing, or genotyping of cell lines.

Swipe to view

ES cell derivation Canadian academic International academic
From mice housed at TCP (Fee based on 3 mutant males & females & expansion of 5 clones) /attempt $1,200.00 $1,920.00

Rederivation of Mouse Strains

Fees do not include genotyping of rederived offspring or animal import, export and shipping charges.

Swipe to view

Service Canadian academic International academic
Housing imported mutant males & technical services in TCP containment (Fee based on 3 cages for 4 months) $414.00 $662.40
Rederivation attempt (Fee based on using C57BL/6N females from TCP breeding colony as embryo donors, mating to 3 mutant males & transfer to 4 recipients) $885.00 $1,416.00