The Centre for Phenogenomics
The Centre for Phenogenomics

FAQs

General

  • What does TCP stand for?

    The Centre for Phenogenomics (formerly "Toronto Centre for Phenogenomics").

  • What is TCP?

    The Centre for Phenogenomics is a $69 million, state-of-the-art facility that enables groundbreaking research and discovery with the goal of advancing human health. Using mouse models, the TCP seeks cures and treatments in areas such as diabetes, cancer, musculoskeletal disease, neurodegenerative disorders, cardiovascular and renal disease, and stem cell and regenerative medicine. TCP is jointly owned and operated by Mount Sinai Hospital and The Hospital for Sick Children and serves local, national and international users.

  • Where is TCP located?

    TCP is located at 25 Orde Street, in the heart of Toronto’s Discovery District, (close to University Avenue and College Street).

  • When was the building construction finished?

    Construction was completed in July 2007

  • Where did the funds come from to build TCP?

    Construction of TCP was funded by the Canada Foundation for Innovation (CFI), the Ontario Innovation Trust (OIT), Mount Sinai Hospital, The Hospital for Sick Children and in-kind funding from industry.

  • How big is the building?

    TCP is the largest vivarium in Canada with 110,000 gross sq ft of custom-designed laboratory space on four floors: two below ground and two above.

  • Why use mouse models?

    Mice are the most efficient, cost-effective and easily genetically manipulated mammalian research model. With over 95% genetic similarity to humans, mice are ideal for modelling human diseases and drug efficacy testing.

  • What is TCP LIMS?

    TCP LIMS is our custom laboratory information management system that allows you to request cost estimates, make service requests, submit mouse line passports, manage your cages and mice, view AUPs, SOPs, reports and forms , book equipment and safety training among many other things. To create an account go to LIMS and click the “Register New User” link. Note that if you are not the Principal Investigator (PI), either have your PI sign up for the account, listing you in his/her contact information OR set up an account on your PI's behalf, listing yourself as the contact person.

  • How do I create a LIMS account?

    To create an account go to LIMS and click the “Register New User” link. Note that if you are not the Principal Investigator (PI), either have your PI sign up for the account OR set up an account on your PI's behalf, listing yourself as the contact person.

Model Production

Animal Holding

  • How do I get a project started at TCP animal facility?

    To work with mice at TCP you will first need a study plan and an approved Animal Use Protocol. Please contact the Services Coordinator at services@phenogenomics.ca to learn more about how to start a project at TCP.

  • Is TCP animal facility accredited?

    TCP adheres to the Canadian Council on Animal Care (CCAC) “Care and Use of Experimental Animals Guidelines.” TCP has been granted the status of Compliance and maintains a current CCAC Certificate of GAP – Good Animal Practice®. We also maintain a current Certificate of Registration of Research Facility under the Animals for Research Act and Regulations granted by the Ontario Ministry of Agriculture, Food and Rural Affairs.

  • Who takes care of the animals at TCP?

    TCP is staffed with both full-time and part-time animal care staff (Veterinary Technicians and Animal Care Attendants) many of whom are certified by the Canadian Association for Laboratory Animal Science (CALAS). There is a full time Veterinarian at TCP who has specialized training in the care and use of animals for research and is board certified by the American College of Laboratory Animal Medicine (ACLAM). All animals are observed on a daily basis by trained Animal Care Attendants for signs of illness, injury or abnormal behaviour.

  • How are the mice housed at TCP?

    TCP strictly ensures that all animals are appropriately housed in compliance with the CCAC Guidelines and the OMAFRA Animals for Research Act. Mice are housed in individually ventilated cages and are fed a standard, high quality, commercially available diet that is irradiated off-site. Water is delivered to the animals through an automated system and undergoes reverse osmosis, acidification and UV irradiation to prevent microbial contamination. All mice in the facility receive environmental enrichment to improve their well-being by encouraging species-specific behaviour. All animal rooms are supplied with 100% fresh air and receive 10-15 air changes per hour. Room temperature is maintained at 20-22⁰C.

  • What is the health status of the mice at TCP?

    TCP is a bioexclusion mouse barrier facility and the mice are specific pathogen free (SPF). TCP operates an animal health surveillance program for all rodent colonies. “Sentinel animals" are maintained for this purpose, and regular serology, PCR, parasite and bacteriology evaluations are conducted to ensure all animals maintain a normative health status.

  • Can I transfer mice into TCP animal facility?

    TCP is a specific pathogen free (SPF) facility. For these reasons, mice from other institutions must be rederived into the facility. For rederivation services see Rederivation section of Model Production. TCP will accept mice from approved commercial suppliers who meet the SPF requirements of the facility.

  • Can I bring a visitor into TCP animal facility?

    TCP is a secure animal facility and visitor access is limited. Visitors may be approved by management on a case-by-case basis. Please contact Betty-Jo Edgell at bettyjo.edgell@phenogenomics.ca to explain the nature of the visit.

  • How do I get a TCP Access Card? I have a new lab member starting. What do they need to do to get an access card?

    To obtain a TCP access card you must complete and submit the Request for TCP Security Access Card form listed here . Access will be granted to those users who have completed the required training courses.

  • Does my TCP access card work on weekends and/or holidays?

    TCP is a secure facility with entry via electronic access card only. Access cards are active from 6:00am – 8:00pm, seven days a week, including holidays. If access is required outside of the times specified, please contact the Animal Care Coordinator at acc@phenogenomics.ca

  • How do I make changes to my current Animal Use Protocol (AUP)?

    Changes to AUP documents are requested by filling out the appropriate forms . The changes will be reviewed by the veterinarian and/or TCP Animal Care Committee and you will be notified once the changes have been approved.

  • Who do I contact to order mice for my research project?

    To order mice you must complete and submit this form . Forms must be submitted before noon on Wednesday to ensure delivery the following week. Mouse strains must be listed on an approved AUP before they can be ordered.

  • How do I schedule technical assistance?

    TCP veterinary technicians can provide a variety of services to assist you with the following: colony maintenance, breeding and weaning, ear notching, tissue sampling for genotyping, blood sampling, surgical procedures, compound delivery (intraperitoneal, subcutaneous, intravenous injections), and oral gavage. Please contact Brianna Attanasio at Brianna.Attanasio@phenogenomics.ca to discuss and schedule the services you require.

  • Who do I contact for specialized training?

    TCP offers customized training for a variety of surgical and technical procedures. Please contact Brianna Attanasio at Brianna.Attanasio@phenogenomics.ca to discuss the training you require and we will schedule a date.

  • How are service requests prioritized?

    We prioritize all service requests on a "first-come, first-served" basis.

Pathology

Cryopreservation & Recovery

  • What is the CMMR?

    The Canadian Mouse Mutant Repository, CMMR, is Canada’s national mouse cryopreservation and recovery facility. It is located in The Centre for Phenogenomics and is dedicated to the preservation and distribution of mutant mouse lines, samples, and ES cells.

  • How do I deposit my mutants and samples in the CMMR?

    The Depositing Investigator (DI) should start the process by registering for an account in TCP LIMS. IF desired, his/her designate can also establish an account in the DI’s name (with the designate as the Contact) and provide the information requested for the completion of the service request. The first step is to create a Mouse line passport for the strain(s) to be deposited in TCP LIMS. These passports provide information about the derivation, specific mutation(s), genetic background, genotyping protocol(s), coat colour, phenotype and health status of the strain. Guidelines naming transgenic and mutant strains can be found in Rülicke et al. (2007) Laboratory Animals 41:301-311 or http://www.informatics.jax.org/mgihome/nomen/index.shtml . Mouse line passport information will be checked for accuracy and completeness.

    Once a Mouse line Passport has been completed, the DI or designate should complete the appropriate service request form in TCP LIMS. Once a service request is submitted, a Material Transfer Agreement (MTA) provided by the CMMR is executed between The Hospital for Sick Children (the legal entity of the CMMR) and the DI and/or his/her institution to clearly outline the obligations of each party and disposition of samples. Once the MTA is complete, the service will be scheduled. The CMMR will receive, process, cryopreserve, and archive the mutants or samples per our fee schedule. The DI will have the option of making his/her mutants or samples available to other investigators.

  • What method of cryopreservation should I choose?

    The method of cryopreservation depends on the specific mutation(s) in your mouse lines, the genetic background, and the fertility and fecundity of the line.

    Sperm cryopreservation cryopreserves only the male germline and is a terminal procedure. Choose Sperm Cryo when you can recover the line using commercially available donor oocytes. At least one generation of subsequent breeding will be required to generate homozygous mice.

    Speed embryo cryopreservation cryopreserves the mutation(s) from the male germline after IVF with wild-type donor oocytes. Choose Speed Cryo when you want to dedicate a small number of mice from your colony for cryopreservation, you do or can maintain your mutation(s) of interest on a commercially available background, and you can accept one generation of mating after cryorecovery to produce homozygous mutant mice.

    Basic embryo cryopreservation cryopreserves both the male and female germlines using embryos from naturally mated males and females from a given mouse line. This method enables the preservation of specific genetic backgrounds, homozygous alleles and/or strains with multiple alleles that would segregated if bred to wild-type mice.

    Choose Basic Cryo when you have good fertility and plug rates in your colony and have at least 8 stud males to dedicate to mating for embryo harvest. While females will be transferred for embryo harvest, males may be maintained in the Requester’s colony, provided females are available there. Basic Cryo may also be performed with wild-type females when males cannot be euthanized for the purposes of cryopreservation.

  • What mutants are available at the CMMR?

    Investigators can search our catalogue of mice and ES cells http://www.cmmr.ca/gene-list.php . Detailed information about gene mutations, alleles, or genetic background can be found through MGI and the International Mouse Phenotyping Consortium .

  • How do I withdraw from the CMMR?

    The Withdrawing Investigator or Institution (WI) initiates the process by registering for an account in TCP LIMS. Once registered, the WI completes a Service Request form to recover or withdraw their mutant of interest. If necessary, a Material Transfer Agreement (MTA) provided by the CMMR will be sent to the WI for completion prior to shipment of samples. The MTA describes the conditions associated with the use of mutants and samples and confirms the rights of the Depositing Investigator/Institution (DI).

    Frozen samples are normally shipped on dry ice or in dry shippers via overnight courier at the WI’s expense. Dry shippers must be returned to the CMMR as soon as possible, at the WI’s expense, to enable their repeated use. Requests for live animals require recovery from cryopreserved samples (if applicable) and coordination of animal shipments between the CMMR and the WI’s animal facility.

  • Who can withdraw samples?

    Most recipients will be associated with a recognized biomedical or genetics research institution. Depositing Investigators and/or their institutions may restrict the distribution of their strains and samples.

  • What conditions may apply?

    The Depositing Investigator or Institution (DI) retains ownership of the sample(s). The CMMR acts with the DI to distribute sample(s) and notifies the DI before releasing samples from the archive. The DI may prohibit access to an archived sample. All DIs will receive updated inventories of their mutants and samples at the CMMR, along with a list of all Withdrawing Investigators/Institutions (WIs) who have received their mutants and/or samples. The DI will be responsible for the authenticity of the mutants and samples and any relevant documentation.

    It is the responsibility of the WI to ensure that any executed MTAs enable their research goals and to obtain appropriate licenses for any relevant patents or other uses. Further it is the responsibility of the WI to ensure that the WI signatory(ies) on any MTA has(have) the legal authority to sign the MTA.

    International WIs must conform to their country’s rules and regulations for importing or exporting biological samples and/or live animals. It is the WI’s responsibility to inform the CMMR of any regulations pertaining to or special documentation required for the shipment of requested samples or animals.

    All WIs must agree to conform to the relevant governing regulations concerning the use of biological reagents and the ethical treatment of animals.

  • How can I access mutants and samples for other repositories?

    Investigators can order mice from virtually any repository. A good place to start your search is MGI and the International Mouse Strain Resource . Samples are shipped from the originating repository to the CMMR for recovery as live mice and distribution to the Requesting Investigator. Shipping gametes and cells across international borders is often less restricted than shipping live mice. The CMMR has considerable experience in the recovery of mutant mice from both sperm and embryos.

  • How are service requests prioritized?

    We prioritize all service requests on a "first-come, first-served" basis.